Original Article
Molecular
Identification of Sudanese Females’ Vaginal Microbiota and Microbiota
associated with
Bacterial Vaginosis, Khartoum, Sudan, 2018
1Research and Development Center Alfajr Collage,
Khartoum – Sudan
2 Departments of Histopathology and Cytology; Medical
Laboratory Science Program, Alfajr College for Science and Technology,
Khartoum, Sudan.
3Faculty of Medical Laboratories,
National Ribat University, Khartoum. Sudan.
*Corresponding Author:
Deena
Abdalfatah Abdelgadir Mohamed, Assistant professor, Research and Development
Center, Alfajr Collage for Science and Technology, Khartoum, Sudan.
Email:
deenamohammed@gmail.com
Abstract
Background: The
microbiota of healthy women changes from birth to menopause. During their
reproductive years, females’ normal flora is dominated by Lactobacillus
species. Females who are identified with vaginal infections are characterized
by lacking Lactobacillus species and the vagina would be dominated by
facultative and strictly anaerobic bacteria. Recently, a new detailed
description of the vaginal microbiome has been attained using quantitative,
real-time Polymerase Chain Reaction (PCR) targeting key bacterial genomes. In
this study, we have used 16S Ribonucleic acid (RNA) sequencing to identify
the Sudanese flora and flora associated with bacterial vaginosis within females
in their reproductive age.
Materials and Methods: This is a descriptive cross-sectional and molecular laboratory-based study; carried out in one reproductive health Centre and two health care facilities in Khartoum State, Sudan. Hundred vaginal swab samples were collected from the participants who visited the Centres. Out of the 100 serial vaginal swabs, 11 samples were randomly selected. The DNA was then extracted from the swab samples and 16S RNA sequencing was performed. The samples were classified into four groups.
Results: Lactobacillus
species was found to be the most dominant organism in all four groups except
for the group with bacterial vaginosis (BV) which was found to be dominated by
the genus Gardnrella and Lactobacillus inners. Factors such as
usage of contraceptives and some Sudanese traditions like circumcision and skin
smoking (Dukhan) have significant effect on the flora (p-value 0.045and 0.03
respectively)
Conclusions:
Sudanese female flora is dominated mainly by Lactobacillus species among
normal and asymptomatic females, while Gardnrellla sp. was dominant
among females with BV. This is consistent with the global picture despite the
differences in customs and traditions of Sudanese women, which was expected to
affect the types of vaginal bacteria. Further studies and metagenomic sequencing
are needed for better identification.
Key words: Bacterial Vaginosis, Microbiota, 16S RNA, Lacbacillus
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